PEER-REVIEWED RESEARCH

Understanding CSCF Syndrome

A comprehensive overview of Cardiospondylocarpofacial Syndrome based on published medical research. All information is drawn from peer-reviewed journals and case studies.

GeneticsSymptomsDiagnosisCardiacSkeletalCase StudiesResearch

Genetic Basis

Cardiospondylocarpofacial syndrome (CSCFS; OMIM #157800) is caused by heterozygous pathogenic variants in the MAP3K7 gene, located on chromosome 6q15. The gene encodes TGF-β-activated kinase 1 (TAK1), a serine-threonine protein kinase that is widely expressed and participates in multiple molecular and cellular processes.

TAK1 forms a complex with specific binding proteins and modulates downstream effectors including c-Jun amino-terminal kinases (JNKs), extracellular-signal regulated kinases (ERKs), p38 MAP kinase, and nuclear factor-κB — affecting a wide range of cellular processes including cell growth and differentiation, immune function, stress responses, and apoptosis.

The nearly ubiquitous expression of TAK1 and the early lethality of knockout mice speak to the critical importance of this protein in embryonic development. In CSCFS, MAP3K7 variants have been shown to decrease TAK1 autophosphorylation and disrupt downstream TAK1-dependent signaling pathways — a key distinction from the related condition Frontometaphyseal Dysplasia Type 2 (FMD2), where variants cause elevated phosphorylation.

CSCFS follows an autosomal dominant inheritance pattern. All known variants are heterozygous and de novo in the majority of reported cases. To date, all small in-frame deletions and splice variants have been associated exclusively with CSCFS, whereas missense variants can cause either CSCFS or FMD2 depending on their specific location and effect on kinase activity.

Key Gene Facts

  • Gene: MAP3K7
  • Chromosome: 6q15
  • Protein: TAK1 (TGF-β-activated kinase 1)
  • Inheritance: Autosomal dominant
  • OMIM: #157800
  • TAK1 effect: Decreased autophosphorylation

Related Condition

Frontometaphyseal Dysplasia 2 (FMD2) is caused by different variants in the same MAP3K7 gene, but causes elevated TAK1 phosphorylation. The two conditions share some features but can be clinically distinguished.

Sources: Le Goff et al., Am J Hum Genet 2016; AbuBakr et al., Cold Spring Harb Mol Case Stud 2020; Nyuzuki et al., Hum Genome Var 2024

Clinical Features & Symptoms

CSCFS can present differently in each individual. Not every person will have all features. Severity varies widely across reported cases.

Cardiovascular

  • Valvular heart disease (mitral, aortic, pulmonary, tricuspid)
  • Septal defects (VSD, ASD, PFO)
  • Bicuspid aortic valve
  • Aortic arch abnormalities
  • Pulmonary artery stenosis
  • Dilated cardiomyopathy (DCM) — newly reported

Skeletal & Spinal

  • Extensive posterior cervical vertebral fusion (synostosis)
  • Thoracic vertebral fusion — can inhibit lung growth
  • Scoliosis
  • Carpal and tarsal bone fusion
  • Brachydactyly (short fingers/toes)
  • Short stature / delayed bone age
  • Pectus excavatum
  • Joint laxity (hypermobility)
  • Elbow flexion contractures

Craniofacial Features

  • Prominent forehead with bitemporal narrowing
  • Hypertelorism (widely spaced eyes)
  • Downslanted palpebral fissures
  • Ptosis
  • Broad nasal bridge with anteverted nares
  • Long philtrum
  • Full cheeks
  • Low-set, posteriorly rotated ears
  • Strabismus
  • High, narrow palate

Hearing & Ears

  • Conductive hearing loss (bilateral)
  • Inner ear malformation
  • Stenosis of the external auditory canal
  • Recurrent otitis media
  • Tympanostomy tube procedures often required

Developmental

  • Mild global developmental delay (gross and fine motor)
  • Speech delay
  • Hypotonia (low muscle tone)
  • Mild intellectual disability in some cases
  • Normal brain MRI in most cases reported

Gastrointestinal

  • Feeding difficulties from birth
  • Gastroesophageal reflux
  • Gastrointestinal dysmotility
  • Failure to thrive
  • Gastrostomy tube (G-tube) feeding in some cases
  • Intestinal obstruction — newly reported

Skin & Integumentary

  • Soft, velvety skin
  • Wrinkled skin of palms and soles
  • Skin laxity

Pulmonary & Immune

  • Recurrent upper and lower respiratory infections
  • Pneumonia episodes
  • Respiratory dysfunction in severe cases
  • Possible immune dysregulation
  • Inflammatory bowel disease reported in one case
Sources: AbuBakr et al. 2020; Nyuzuki et al. 2024; Das & Criscuolo, Ann Pediatr Cardiol 2025; Yang et al., Front Pediatr 2025

Diagnosis

01

Clinical Recognition

Recognition of characteristic facial features, skeletal abnormalities, and cardiac findings. Many patients are initially misdiagnosed with Noonan syndrome or other RASopathies due to overlapping features.

02

Ruling Out Other Conditions

Prior negative genetic testing commonly includes karyotype, chromosomal microarray, PTPN11 sequencing, fragile X, and subtelomeric FISH. CSCFS is frequently a diagnosis reached after exclusion of more common syndromes.

03

Whole Exome Sequencing (WES)

Whole exome sequencing of the patient and both parents (trio-WES) is currently the most effective method for identifying MAP3K7 variants. WES has identified all genetically confirmed CSCFS cases in the literature.

04

Sanger Confirmation

Identified variants are confirmed by Sanger sequencing. De novo variants are confirmed by verifying absence of the variant in both biological parents.

05

Variant Classification

Variants are classified using ACMG/AMP guidelines. All confirmed CSCFS variants are located in the kinase domain of MAP3K7. De novo status (PS2), rarity in controls (PM2), and protein length/function changes (PM4) support pathogenicity.

Diagnostic Challenges

  • Extremely low prevalence means most clinicians have never encountered a case
  • Significant phenotypic overlap with Noonan syndrome and FMD2 complicates clinical diagnosis
  • Presentation varies widely — some features become more apparent with age
  • Fewer than 30 genetically confirmed cases worldwide as of 2025
  • No single pathognomonic feature — diagnosis requires integration of multiple clinical findings

When to Suspect CSCFS

Consider CSCFS in a child presenting with two or more of the following:

  • Congenital heart disease (especially valvular)
  • Characteristic facial features
  • Cervical or thoracic vertebral fusion
  • Carpal or tarsal bone fusion
  • Conductive hearing loss
  • Failure to thrive / short stature
  • Brachydactyly with joint laxity
Sources: AbuBakr et al. 2020; Das & Criscuolo 2025; Yang et al. 2025; Le Goff et al. 2016

Published Case Studies

Fewer than 30 genetically confirmed CSCFS cases exist in medical literature worldwide. Each case contributes critical knowledge about this ultra-rare condition.

Patient

6 patients across 2 families + 2 unrelated individuals

Genetic Variant

In-frame 3-bp deletions; missense variants W241R and G110C in MAP3K7

Outcome

Survivors — father and adult relatives included; one infant death from diaphragmatic hernia complications

Key Findings

  • First paper to genetically define CSCFS as caused by MAP3K7 mutations
  • Described the core clinical phenotype: facial dysmorphism, short stature, carpal-tarsal fusion, vertebral synostosis, cardiac defects
  • CSCFS variants decrease TAK1 autophosphorylation (vs. FMD2 which increases it)
  • Autosomal dominant inheritance confirmed
  • No cardiomyopathy reported in any of the 6 cases

Cited References

1

Le Goff C, Rogers C, Le Goff W, et al. Heterozygous mutations in MAP3K7, encoding TGF-β-activated kinase 1, cause cardiospondylocarpofacial syndrome. Am J Hum Genet. 2016; 99:407–413.

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2

AbuBakr F, Jeffries L, Ji W, McGrath JM, Lakhani SA. A novel variant in MAP3K7 associated with an expanded cardiospondylocarpofacial syndrome phenotype. Cold Spring Harb Mol Case Stud. 2020; 6(3):a005207.

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3

Morlino S, Castori M, Dordoni C, et al. A novel MAP3K7 splice mutation causes cardiospondylocarpofacial syndrome with features of hereditary connective tissue disorder. Eur J Hum Genet. 2018; 26:582–586.

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4

Nyuzuki H, Ozawa J, Nagasaki K, et al. A severe case of cardiospondylocarpofacial syndrome with a novel MAP3K7 variant. Hum Genome Var. 2024; 11:8.

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5

Das BB, Criscuolo JJ. Neonatal dilated cardiomyopathy and cardiospondylocarpofacial syndrome linked to a novel MAP3K7 gene mutation. Ann Pediatr Cardiol. 2025; 18(1):68–71.

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6

Yang Q, Zhang Q, Yi S, et al. Genetic diagnosis and clinical characteristics analysis of cardiospondylocarpofacial syndrome in a Chinese family. Front Pediatr. 2025; 13:1651803.

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7

Shepherd WB, Colaiacovo S, Campbell C, Saleh M. A novel MAP3K7 mutation in a child with cardiospondylocarpofacial syndrome and orofacial clefting. Clin Genet. 2023; 103:254–255.

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Important note: The information on this page is intended for educational purposes to help families, caregivers, and medical professionals understand CSCFS. It is not a substitute for professional medical advice. Always consult a qualified geneticist or specialist for diagnosis and treatment decisions.